Quantitative microbial risk assessment of Clostridium perfringens in beef jerky (2024)

Abstract

We developed a quantitative microbial risk assessment model for determning the effect of seasoning on Clostridium perfringens behavior in beef jerky under aerobic and anaerobic conditions. C. perfringens was not detected (<0.5 log CFU/g) in beef jerky samples (n=275), regardless of storage conditions or the presence of seasoning. Survival models of C. perfringens on beef jerky were developed as a function of temperature (10, 17, 25, and 35 o C). Risk of C. perfringens due to the consumption of beef jerky was estimated with @RISK and FDA-iRISK. The probability of foodborne illness due to C. perfringens through consumption of seasoned, vacuum packed beef jerky was estimated to be 2.77×10 -16 per person per day. Overall, the risk of contamination of beef jerky with C. perfringens is very low.

Original languageEnglish
Pages (from-to)621-628
Number of pages8
JournalKorean Journal of Food Science and Technology
Volume50
Issue number6
DOIs
Publication statusPublished - 2018

Bibliographical note

Publisher Copyright:
©The Korean Society of Food Science and Technology

Keywords

  • Aerobic and vacuum packing
  • Beef jerky
  • Microbial risk assessment
  • Predictive model of C. perfringens
  • Seasoning

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Nam, G. W., Kim, S. J. (2018). Quantitative microbial risk assessment of Clostridium perfringens in beef jerky. Korean Journal of Food Science and Technology, 50(6), 621-628. https://doi.org/10.9721/KJFST.2018.50.6.621

Nam, Gun Woo ; Kim, Su Jin ; Yoon, Ki Sun. / Quantitative microbial risk assessment of Clostridium perfringens in beef jerky. In: Korean Journal of Food Science and Technology. 2018 ; Vol. 50, No. 6. pp. 621-628.

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abstract = " We developed a quantitative microbial risk assessment model for determning the effect of seasoning on Clostridium perfringens behavior in beef jerky under aerobic and anaerobic conditions. C. perfringens was not detected (<0.5 log CFU/g) in beef jerky samples (n=275), regardless of storage conditions or the presence of seasoning. Survival models of C. perfringens on beef jerky were developed as a function of temperature (10, 17, 25, and 35 o C). Risk of C. perfringens due to the consumption of beef jerky was estimated with @RISK and FDA-iRISK. The probability of foodborne illness due to C. perfringens through consumption of seasoned, vacuum packed beef jerky was estimated to be 2.77×10 -16 per person per day. Overall, the risk of contamination of beef jerky with C. perfringens is very low.",

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language = "English",

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Nam, GW, Kim, SJ 2018, 'Quantitative microbial risk assessment of Clostridium perfringens in beef jerky', Korean Journal of Food Science and Technology, vol. 50, no. 6, pp. 621-628. https://doi.org/10.9721/KJFST.2018.50.6.621

Quantitative microbial risk assessment of Clostridium perfringens in beef jerky. / Nam, Gun Woo; Kim, Su Jin; Yoon, Ki Sun.
In: Korean Journal of Food Science and Technology, Vol. 50, No. 6, 2018, p. 621-628.

Research output: Contribution to journalArticlepeer-review

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T1 - Quantitative microbial risk assessment of Clostridium perfringens in beef jerky

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AU - Kim, Su Jin

AU - Yoon, Ki Sun

N1 - Publisher Copyright:©The Korean Society of Food Science and Technology

PY - 2018

Y1 - 2018

N2 - We developed a quantitative microbial risk assessment model for determning the effect of seasoning on Clostridium perfringens behavior in beef jerky under aerobic and anaerobic conditions. C. perfringens was not detected (<0.5 log CFU/g) in beef jerky samples (n=275), regardless of storage conditions or the presence of seasoning. Survival models of C. perfringens on beef jerky were developed as a function of temperature (10, 17, 25, and 35 o C). Risk of C. perfringens due to the consumption of beef jerky was estimated with @RISK and FDA-iRISK. The probability of foodborne illness due to C. perfringens through consumption of seasoned, vacuum packed beef jerky was estimated to be 2.77×10 -16 per person per day. Overall, the risk of contamination of beef jerky with C. perfringens is very low.

AB - We developed a quantitative microbial risk assessment model for determning the effect of seasoning on Clostridium perfringens behavior in beef jerky under aerobic and anaerobic conditions. C. perfringens was not detected (<0.5 log CFU/g) in beef jerky samples (n=275), regardless of storage conditions or the presence of seasoning. Survival models of C. perfringens on beef jerky were developed as a function of temperature (10, 17, 25, and 35 o C). Risk of C. perfringens due to the consumption of beef jerky was estimated with @RISK and FDA-iRISK. The probability of foodborne illness due to C. perfringens through consumption of seasoned, vacuum packed beef jerky was estimated to be 2.77×10 -16 per person per day. Overall, the risk of contamination of beef jerky with C. perfringens is very low.

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Nam GW, Kim SJ, Yoon KS. Quantitative microbial risk assessment of Clostridium perfringens in beef jerky. Korean Journal of Food Science and Technology. 2018;50(6):621-628. doi: 10.9721/KJFST.2018.50.6.621

Quantitative microbial risk assessment of Clostridium perfringens in beef jerky (2024)

FAQs

What is a quantitative microbial risk assessment? ›

Quantitative microbial risk assessment (QMRA) is a mathematical modeling approach used to estimate the risk of infection and illness when a population is exposed to microorganisms in the environment.

How to test for Clostridium perfringens? ›

C. perfringens food poisoning is diagnosed when a laboratory test detects the bacteria or toxin in a patient's stool (poop) sample or the bacteria are found in food linked to the illness. But most people with C. perfringens food poisoning do not receive a diagnosis.

What is risk characterization in QMRA? ›

Risk characterization.

Risk characterization is often conducted using Monte Carlo simulations, which help account for variability and uncertainty in estimated health risks. Monte Carlo simulations also allow for a sensitivity analysis that can identify risk factors (environmental factors, exposure variables, etc.)

What is the limit of Clostridium perfringens in food? ›

In addition, this assay provides test results less than 24 h, significantly superior to standard culture-based method. Based on the food safety of international criteria, the limit for C. perfringens in ready-to-eat food ranges from < 10 CFU/g [28], < 20 CFU/g [29], < 100 CFU/g [30, 31] and 100–1,000 CFU/g [32].

What is the quantitative risk assessment example? ›

Quantitative risk analysis is an objective, data-driven process to evaluate the impact of an identified risk on the overall project plan. Decision tree analysis, estimated monetary value, three-point estimate, and Monte Carlo analysis are examples of quantitative risk assessment.

What is a quantitative risk assessment for food safety? ›

Quantitative risk assessment is a scientific process by which the relationship between levels of exposure to a noxious agent and the lifetime incidence rates of an undesirable consequence is determined.

What method is used to detect Clostridium perfringens? ›

DNA is extracted from the RPM subculture, and then analyzed for the presence of C. perfringens toxin genes via polymerase chain reaction (PCR). Depending on the type of food sampled, typically 15-20% of samples test positive for C. perfringens.

What is the rapid test for Clostridium perfringens? ›

PERFRINGENS ENTEROTOXIN TEST is a rapid ELISA for the detection of Clostridium perfringens enterotoxin in fecal specimens. The test is an alternative to tissue culture assay for detecting the enterotoxin in fecal specimens.

What is C. perfringens in the food industry? ›

Clostridium perfringens bacteria are one of the most common causes of foodborne illness (food poisoning). CDC estimates these bacteria cause nearly 1 million illnesses in the United States every year. C. perfringens can be found on raw meat and poultry, in the intestines of animals, and in the environment.

How do you measure risk quantitatively? ›

Steps in the quantitative risk analysis process
  1. Identify areas for uncertainty. ...
  2. Assess the costs of each risk. ...
  3. Determine the probability of each risk occurring. ...
  4. Calculate the expected cost of each potential risk.
Jun 24, 2022

What is a quantification of risk assessment? ›

Quantitative risk assessment refers to both applying risk quantification to individual risks, and the process for applying it methodically across your entire risk register to get a full picture of the financial impact spread out across your organization and to start prioritizing the risks that carry the highest ...

Which best describes a quantitative risk analysis? ›

It uses numerical values and complex data to determine the probability of a specific event and the potential impact that event could have on the organization. This method involves collecting data about specific, measurable quantities of risk using mathematical models and simulations to analyze them.

What foods are high risk for Clostridium perfringens? ›

Clostridium perfringens
  • C. perfringens can cause gastro illness.
  • Anyone can get sick with C. ...
  • Foods at higher risk of contamination include meat and poultry, thickened sauces (gravies, etc) and pre-cooked foods, especially spiced and herbed dishes.
Mar 18, 2024

What is the danger zone for Clostridium perfringens? ›

Foodborne illness from C. perfringens primarily occurs when food is kept in the temperature danger zone (between 5°C and 60°C) for too long - commonly, if cooling or reheating cooked food is not achieved rapidly.

What are the statistics of Clostridium perfringens? ›

There are an estimated 177,000 infections of Clostridium perfringens each year in Canada. Clostridium perfringens outbreaks are common in Canada and most frequently occur in a local setting. They often happen at community events and in institutions, such as: hospitals.

What is an example of a QMRA? ›

QMRA uses a mathematical model to estimate risk. For example, QMRA can be used to estimate the likelihood that someone would get sick from microorganisms in water they drink, irrigation water spray they breathe in, food they eat, water they swim in, as well as other exposure scenarios.

What best describes a quantitative risk assessment? ›

NIST describes quantitative risk as the “use of a set of methods, principles, or rules for assessing risks based on the use of numbers where the meanings and proportionality of values are maintained inside and outside the context of the assessment.”

What is microbiological risk assessment? ›

Microbiological risk assessment (MRA) is a structured process for determining the public health risk associated with biological hazards in a food. It includes hazard identification, exposure assessment, hazard characterization, and risk characterization.

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