Abstract
This study aimed to explore efficient DNA extraction methods using tree nuts. Four different DNA extraction procedures, including silica membrane method, modified silica method, cetyltrimethylammonium bromide (CTAB) method, and modified CTAB method were examined for their relative efficiency in extracting DNA from pistachio, pine nut, almond, hazelnut, cashew nut, walnut, and peanut. The quality and quantity of the extracted DNA were subsequently assessed by spectrometric measurements, gel electrophoresis, and PCR amplifications. CTAB method was the most appropriate one for extracting DNA from pine nut, cashew nut, pistachio, and peanut. However, it could be replaced by the silica membrane method for walnut and modified CTAB method for almond and hazelnut.
| Original language | English |
|---|---|
| Pages (from-to) | 357-361 |
| Number of pages | 5 |
| Journal | Korean Journal of Food Science and Technology |
| Volume | 50 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 2018 |
Bibliographical note
Publisher Copyright:
© The Korean Society of Food Science and Technology.
Keywords
- Cetyltrimethylammonium bromide
- DNA extraction
- PCR
- Silica
- Tree nuts
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Suh, S. M., Park, S. B., Kim, M. J. (2018). Comparison of methods of DNA extraction from tree nuts. Korean Journal of Food Science and Technology, 50(4), 357-361. https://doi.org/10.9721/KJFST.2018.50.4.357
Suh, Seung Man ; Park, Saet Byul ; Kim, Mi Ju et al. / Comparison of methods of DNA extraction from tree nuts. In: Korean Journal of Food Science and Technology. 2018 ; Vol. 50, No. 4. pp. 357-361.
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title = "Comparison of methods of DNA extraction from tree nuts",
abstract = "This study aimed to explore efficient DNA extraction methods using tree nuts. Four different DNA extraction procedures, including silica membrane method, modified silica method, cetyltrimethylammonium bromide (CTAB) method, and modified CTAB method were examined for their relative efficiency in extracting DNA from pistachio, pine nut, almond, hazelnut, cashew nut, walnut, and peanut. The quality and quantity of the extracted DNA were subsequently assessed by spectrometric measurements, gel electrophoresis, and PCR amplifications. CTAB method was the most appropriate one for extracting DNA from pine nut, cashew nut, pistachio, and peanut. However, it could be replaced by the silica membrane method for walnut and modified CTAB method for almond and hazelnut.",
keywords = "Cetyltrimethylammonium bromide, DNA extraction, PCR, Silica, Tree nuts",
author = "Suh, {Seung Man} and Park, {Saet Byul} and Kim, {Mi Ju} and Kim, {Hae Yeong}",
note = "Publisher Copyright: {\textcopyright} The Korean Society of Food Science and Technology.",
year = "2018",
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doi = "10.9721/KJFST.2018.50.4.357",
language = "English",
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Suh, SM, Park, SB, Kim, MJ 2018, 'Comparison of methods of DNA extraction from tree nuts', Korean Journal of Food Science and Technology, vol. 50, no. 4, pp. 357-361. https://doi.org/10.9721/KJFST.2018.50.4.357
Comparison of methods of DNA extraction from tree nuts. / Suh, Seung Man; Park, Saet Byul; Kim, Mi Ju et al.
In: Korean Journal of Food Science and Technology, Vol. 50, No. 4, 08.2018, p. 357-361.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Comparison of methods of DNA extraction from tree nuts
AU - Suh, Seung Man
AU - Park, Saet Byul
AU - Kim, Mi Ju
AU - Kim, Hae Yeong
N1 - Publisher Copyright:© The Korean Society of Food Science and Technology.
PY - 2018/8
Y1 - 2018/8
N2 - This study aimed to explore efficient DNA extraction methods using tree nuts. Four different DNA extraction procedures, including silica membrane method, modified silica method, cetyltrimethylammonium bromide (CTAB) method, and modified CTAB method were examined for their relative efficiency in extracting DNA from pistachio, pine nut, almond, hazelnut, cashew nut, walnut, and peanut. The quality and quantity of the extracted DNA were subsequently assessed by spectrometric measurements, gel electrophoresis, and PCR amplifications. CTAB method was the most appropriate one for extracting DNA from pine nut, cashew nut, pistachio, and peanut. However, it could be replaced by the silica membrane method for walnut and modified CTAB method for almond and hazelnut.
AB - This study aimed to explore efficient DNA extraction methods using tree nuts. Four different DNA extraction procedures, including silica membrane method, modified silica method, cetyltrimethylammonium bromide (CTAB) method, and modified CTAB method were examined for their relative efficiency in extracting DNA from pistachio, pine nut, almond, hazelnut, cashew nut, walnut, and peanut. The quality and quantity of the extracted DNA were subsequently assessed by spectrometric measurements, gel electrophoresis, and PCR amplifications. CTAB method was the most appropriate one for extracting DNA from pine nut, cashew nut, pistachio, and peanut. However, it could be replaced by the silica membrane method for walnut and modified CTAB method for almond and hazelnut.
KW - Cetyltrimethylammonium bromide
KW - DNA extraction
KW - PCR
KW - Silica
KW - Tree nuts
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U2 - 10.9721/KJFST.2018.50.4.357
DO - 10.9721/KJFST.2018.50.4.357
M3 - Article
AN - SCOPUS:85052867808
SN - 0367-6293
VL - 50
SP - 357
EP - 361
JO - Korean Journal of Food Science and Technology
JF - Korean Journal of Food Science and Technology
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ER -
Suh SM, Park SB, Kim MJ, Kim HY. Comparison of methods of DNA extraction from tree nuts. Korean Journal of Food Science and Technology. 2018 Aug;50(4):357-361. doi: 10.9721/KJFST.2018.50.4.357
