Simple identification method to distinguish Bacillus pumilus from B. zhangzhouensis (2024)

Ko, J. W., Kim, Y. J. (2023). Simple identification method to distinguish Bacillus pumilus from B. zhangzhouensis. Korean Journal of Food Science and Technology, 55(5), 515-522. https://doi.org/10.9721/KJFST.2023.55.5.515

Ko, Jung Woo ; Kim, Ye Jin ; Park, Cheon Seok. / Simple identification method to distinguish Bacillus pumilus from B. zhangzhouensis. In: Korean Journal of Food Science and Technology. 2023 ; Vol. 55, No. 5. pp. 515-522.

@article{aa596c3ded5c46399943de105bc5a32f,

title = "Simple identification method to distinguish Bacillus pumilus from B. zhangzhouensis",

abstract = "Bacillus pumilus and B. zhangzhouensis are two major bacterial strains isolated from Doenjang, a traditional Korean fermented food. However, identification of these species is challenging because of their similarity in the 16s rKNA region. Instead of relying on 16s rRNA sequencing, this study explored the possibility of distinguishing between these two strains based on the morphology of colonies grown on solid media. Four types of media (BHI, BL, PC, and TS) were used to observe the morphological characteristics of colonies. The results showed that it was easy to differentiate B. pumilus and B. zhangzhouensis in BL medium. Therefore, a simple and cost-effective way to analyze these strains was proposed using one-way PCR sequencing of the 16s rRNA region and evaluation of their growth patterns on BL solid medium. In this method, one-way PCR sequencing was useful for distinguishing B. pumilus and B. zhangzhouensis from other Bacillus strains, and the growth pattern in BL medium distinguished B. pumilus from B. zhangzhouensis.",

keywords = "B. pumilus, B. zhangzhouensis, Doenjang, fermented food",

author = "Ko, {Jung Woo} and Kim, {Ye Jin} and Park, {Cheon Seok}",

note = "Publisher Copyright: {\textcopyright} 2023 Korean Society of Food Science and Technology. All rights reserved.",

year = "2023",

doi = "10.9721/KJFST.2023.55.5.515",

language = "English",

volume = "55",

pages = "515--522",

journal = "Korean Journal of Food Science and Technology",

issn = "0367-6293",

publisher = "Tong Kwahak Hoe",

number = "5",

}

Ko, JW, Kim, YJ 2023, 'Simple identification method to distinguish Bacillus pumilus from B. zhangzhouensis', Korean Journal of Food Science and Technology, vol. 55, no. 5, pp. 515-522. https://doi.org/10.9721/KJFST.2023.55.5.515

Simple identification method to distinguish Bacillus pumilus from B. zhangzhouensis. / Ko, Jung Woo; Kim, Ye Jin; Park, Cheon Seok.
In: Korean Journal of Food Science and Technology, Vol. 55, No. 5, 2023, p. 515-522.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Simple identification method to distinguish Bacillus pumilus from B. zhangzhouensis

AU - Ko, Jung Woo

AU - Kim, Ye Jin

AU - Park, Cheon Seok

N1 - Publisher Copyright:© 2023 Korean Society of Food Science and Technology. All rights reserved.

PY - 2023

Y1 - 2023

N2 - Bacillus pumilus and B. zhangzhouensis are two major bacterial strains isolated from Doenjang, a traditional Korean fermented food. However, identification of these species is challenging because of their similarity in the 16s rKNA region. Instead of relying on 16s rRNA sequencing, this study explored the possibility of distinguishing between these two strains based on the morphology of colonies grown on solid media. Four types of media (BHI, BL, PC, and TS) were used to observe the morphological characteristics of colonies. The results showed that it was easy to differentiate B. pumilus and B. zhangzhouensis in BL medium. Therefore, a simple and cost-effective way to analyze these strains was proposed using one-way PCR sequencing of the 16s rRNA region and evaluation of their growth patterns on BL solid medium. In this method, one-way PCR sequencing was useful for distinguishing B. pumilus and B. zhangzhouensis from other Bacillus strains, and the growth pattern in BL medium distinguished B. pumilus from B. zhangzhouensis.

AB - Bacillus pumilus and B. zhangzhouensis are two major bacterial strains isolated from Doenjang, a traditional Korean fermented food. However, identification of these species is challenging because of their similarity in the 16s rKNA region. Instead of relying on 16s rRNA sequencing, this study explored the possibility of distinguishing between these two strains based on the morphology of colonies grown on solid media. Four types of media (BHI, BL, PC, and TS) were used to observe the morphological characteristics of colonies. The results showed that it was easy to differentiate B. pumilus and B. zhangzhouensis in BL medium. Therefore, a simple and cost-effective way to analyze these strains was proposed using one-way PCR sequencing of the 16s rRNA region and evaluation of their growth patterns on BL solid medium. In this method, one-way PCR sequencing was useful for distinguishing B. pumilus and B. zhangzhouensis from other Bacillus strains, and the growth pattern in BL medium distinguished B. pumilus from B. zhangzhouensis.

KW - B. pumilus

KW - B. zhangzhouensis

KW - Doenjang

KW - fermented food

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U2 - 10.9721/KJFST.2023.55.5.515

DO - 10.9721/KJFST.2023.55.5.515

M3 - Article

AN - SCOPUS:85178060385

SN - 0367-6293

VL - 55

SP - 515

EP - 522

JO - Korean Journal of Food Science and Technology

JF - Korean Journal of Food Science and Technology

IS - 5

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Ko JW, Kim YJ, Park CS. Simple identification method to distinguish Bacillus pumilus from B. zhangzhouensis. Korean Journal of Food Science and Technology. 2023;55(5):515-522. doi: 10.9721/KJFST.2023.55.5.515